Search results for " FACS"
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La seduzione squadrista in un fascismo periferico. Memoria e ceto politico a Palermo
2019
During Mussolini's dictatorship, the concept of squadrismo was an essential aspect of the Fascist identity. Squadrismo was widespread in Central and Northern Italy in particular, and certain cities in the South subsequently resorted to fabricating their own squadrist myths and traditions. This essay focuses on the case of Palermo. The relationship between the fascist squads' origins and certain local leaders' life histories is underlined. More specifically, an attempt is made to show how Palermo had more "political" prefects than many other Italian provinces. As Fascism emerged later in the South than elsewhere in Italy, the local party hierarchies had not taken part in the original Fascist…
FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter
2021
Summary Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).